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Cytochrome P450

Name: Cytochrome P450
Brand: Springer-Verlag New York Inc.
SKU: 39319364
Price: 119.54 EUR
Availability: InStock
Author: Zhengyin Yan
ISBN: 9781071615447

In Vitro Methods and Protocols

Zhengyin Yan

Gary W. Caldwell

Language

English

Book Paperback

Libristo code: 39319364

Publishers Springer-Verlag New York Inc., July 2022

This collection explores detailed experimental protocols necessary for setting up a variety of in vi... Full description

Libristo code: 39319364

292 b

119.54 €

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This collection explores detailed experimental protocols necessary for setting up a variety of in vitro cytochrome P450 (CYP) assays that are vital in selecting drug candidates in a drug discovery pipeline. Major factors affecting drug metabolism include CYP expression levels, kinetic parameters for individual CYP enzymes, CYP inhibition and induction, time-dependent inhibition (TDI), CYP stability, non-CYP stability, UDP-glucuronosyltransferases (UGT) stability, excretion mechanisms, and drug-drug interactions (DDI), all addressed in this volume. Written for the Methods in Pharmacology and Toxicology series, chapters include helpful background information on the in vitro assay, a list of all the materials, reagents, and equipment necessary to carry out the assay, a step-by-step protocol, notes containing common and unexpected experimental problems in the assay, as well as references containing important supplementary reading. Authoritative and practical, Cytochrome P450: In Vitro Methods and Protocols serves as a key guide for researchers in the area of discovery and development of new medicines.